C3A Cells-Inoculated Affinity Membrane for Bilirubin Removal

Affinity membranes have the potential to enhance specific adsorption of toxins, however, they suffer from insufficient hemocompatibility and low therapeutic efficiency during blood detoxification therapy. Herein, we combine an affinity membrane with a bioreactor develop purification cell functions. To fabricate membrane, polyethersulfone (iPES) macroporous support layer was prepared by phase inversion technique modified polydopamine (PDA). The iPES/PDA composite exhibited excellent biocompatibility compatibility, as well controllable permeability. Lysine (Lys) hepatocytes (C3A cells), which were selected ligand for bilirubin cells, respectively, immobilized on via active group adhesiveness PDA coating membrane. fabricated C3A cells-inoculated iPES/PDA/Lys not only achieved high activity function inoculated cells but also significantly improved toxin clearance efficiency.